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superscript iii one-step rt-pcr kit  (Thermo Fisher)


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    Thermo Fisher superscript iii one-step rt-pcr kit
    Superscript Iii One Step Rt Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iii+rt-pcr+kit/high+capacity+cdna+reverse+transcription+kit/bio_rxiv__2025__07__11__664278-57-18-21
    Average 90 stars, based on 1 article reviews
    superscript iii one-step rt-pcr kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: Mesenchymal Stem Cell Exosomes as Immunomodulatory Therapy for Corneal Scarring
    Article Snippet: Reverse transcription of total RNA (1 μg) was performed with a Superscript III RT-PCR kit (Thermo Fisher).

    Article Title: Establishment of transgene-free induced pluripotent stem cells reprogrammed from human stem cells of apical papilla for neural differentiation
    Article Snippet: Total RNA was isolated using RNeasy Mini Kit (Qiagen, Vaenicia, CA, USA) from TF-SCAP iPSCs grown on Matrigel with TESR2 medium and neural-like cells after neurogenesis. cDNA was synthesized with a SuperScript III RT-PCR kit (Invitrogen) followed by PCR using Taq DNA Polymerase (Invitrogen) to detect the human neural gene expression listed in Table .

    Article Title: Genome-wide analysis of LysM gene family members and their expression in response to Colletotrichum fructicola infection in Octoploid strawberry( Fragaria × ananassa )
    Article Snippet: Briefly, total RNA was extracted from strawberry leaf samples, which included a mock inoculation and inoculation of the leaf samples at 24, 72, and 96 hpi using RNAiso Plus (TaKaRa, Otsu, Japan), followed by reverse transcription with an oligo(dT) primer using the Superscript III RT-PCR kit (Invitrogen, Carlsbad, CA).

    Article Title: Neopterin production in relation to COVID-19 in the Haut-Ogooué Province, Gabon.
    Article Snippet: The real-time RT-PCR assay was performed using a Superscript III RT-PCR kit Invitrogen in simplex, with primers and probes targeting the envelope coding for the E gene and the RdRP gene coding for RNA-dependent RNA polymerase (Tib-Molbiol kit).

    Article Title: Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations
    Article Snippet: RNA (1 μg) was reverse transcribed into cDNA libraries using Superscript III RT-PCR kit (Invitrogen; 18080-093), and qPCR was performed with QuantStudio3 (Applied Biosystems) using qPCR SYBR Master Mix (Promega; A6001) to detect SYBR Green 1.

    Article Title: Nanoparticle targeting of neutrophil glycolysis prevents lung ischemia-reperfusion injury.
    Article Snippet: Neutrophils exacerbate pulmonary ischemia-reperfusion injury (IRI) resulting in poor short and long-term outcomes for lung transplant recipients.. Glycolysis powers neutrophil activation, but it remains unclear if neutrophil-specific targeting of this pathway will inhibit IRI.. Lipid nanoparticles containing the glycolysis flux inhibitor 2-deoxyglucose (2-DG) were conjugated to neutrophil-specific Ly6G antibodies (NP-Ly6G[2-DG]).

    Article Title: Epidemiology of first cases of SARS-CoV-2 infection, from March to April 2020, in Gabon
    Article Snippet: RT-PCR was performed using Superscript III RT-PCR kit Invitrogen in simplex, primers and probes targeted the E gene coding envelope and the RdRP gene coding RNA-dependent RNA polymerase (kit Tib-Molbiol), in a 7500 Real Time PCR System (Applied Biosystems).

    Article Title: Arginase 1 is a marker of protection against illness in contacts of leprosy patients
    Article Snippet: Standard denaturing agarose gel electrophoresis was performed to determine RNA integrity visualized on a UV transilluminator. cDNA synthesis was carried out using the Superscript III RT-PCR Kit (Applied Biosystems, Branchburg, NJ, USA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Mesenchymal Stem Cell Exosomes as Immunomodulatory Therapy for Corneal Scarring
    Article Snippet: Reverse transcription of total RNA (1 μg) was performed with a Superscript III RT-PCR kit (Thermo Fisher).

    Article Title: Establishment of transgene-free induced pluripotent stem cells reprogrammed from human stem cells of apical papilla for neural differentiation
    Article Snippet: Total RNA was isolated using RNeasy Mini Kit (Qiagen, Vaenicia, CA, USA) from TF-SCAP iPSCs grown on Matrigel with TESR2 medium and neural-like cells after neurogenesis. cDNA was synthesized with a SuperScript III RT-PCR kit (Invitrogen) followed by PCR using Taq DNA Polymerase (Invitrogen) to detect the human neural gene expression listed in Table .

    Article Title: Genome-wide analysis of LysM gene family members and their expression in response to Colletotrichum fructicola infection in Octoploid strawberry( Fragaria × ananassa )
    Article Snippet: Briefly, total RNA was extracted from strawberry leaf samples, which included a mock inoculation and inoculation of the leaf samples at 24, 72, and 96 hpi using RNAiso Plus (TaKaRa, Otsu, Japan), followed by reverse transcription with an oligo(dT) primer using the Superscript III RT-PCR kit (Invitrogen, Carlsbad, CA).

    Article Title: Neopterin production in relation to COVID-19 in the Haut-Ogooué Province, Gabon.
    Article Snippet: The real-time RT-PCR assay was performed using a Superscript III RT-PCR kit Invitrogen in simplex, with primers and probes targeting the envelope coding for the E gene and the RdRP gene coding for RNA-dependent RNA polymerase (Tib-Molbiol kit).

    Article Title: Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations
    Article Snippet: RNA (1 μg) was reverse transcribed into cDNA libraries using Superscript III RT-PCR kit (Invitrogen; 18080-093), and qPCR was performed with QuantStudio3 (Applied Biosystems) using qPCR SYBR Master Mix (Promega; A6001) to detect SYBR Green 1.

    Article Title: Nanoparticle targeting of neutrophil glycolysis prevents lung ischemia-reperfusion injury.
    Article Snippet: Neutrophils exacerbate pulmonary ischemia-reperfusion injury (IRI) resulting in poor short and long-term outcomes for lung transplant recipients.. Glycolysis powers neutrophil activation, but it remains unclear if neutrophil-specific targeting of this pathway will inhibit IRI.. Lipid nanoparticles containing the glycolysis flux inhibitor 2-deoxyglucose (2-DG) were conjugated to neutrophil-specific Ly6G antibodies (NP-Ly6G[2-DG]).

    Article Title: Epidemiology of first cases of SARS-CoV-2 infection, from March to April 2020, in Gabon
    Article Snippet: RT-PCR was performed using Superscript III RT-PCR kit Invitrogen in simplex, primers and probes targeted the E gene coding envelope and the RdRP gene coding RNA-dependent RNA polymerase (kit Tib-Molbiol), in a 7500 Real Time PCR System (Applied Biosystems).

    Article Title: Arginase 1 is a marker of protection against illness in contacts of leprosy patients
    Article Snippet: Standard denaturing agarose gel electrophoresis was performed to determine RNA integrity visualized on a UV transilluminator. cDNA synthesis was carried out using the Superscript III RT-PCR Kit (Applied Biosystems, Branchburg, NJ, USA).

    Expressing:

    Article Title: Mesenchymal Stem Cell Exosomes as Immunomodulatory Therapy for Corneal Scarring
    Article Snippet: Reverse transcription of total RNA (1 μg) was performed with a Superscript III RT-PCR kit (Thermo Fisher).

    Article Title: Establishment of transgene-free induced pluripotent stem cells reprogrammed from human stem cells of apical papilla for neural differentiation
    Article Snippet: Total RNA was isolated using RNeasy Mini Kit (Qiagen, Vaenicia, CA, USA) from TF-SCAP iPSCs grown on Matrigel with TESR2 medium and neural-like cells after neurogenesis. cDNA was synthesized with a SuperScript III RT-PCR kit (Invitrogen) followed by PCR using Taq DNA Polymerase (Invitrogen) to detect the human neural gene expression listed in Table .

    Article Title: Genome-wide analysis of LysM gene family members and their expression in response to Colletotrichum fructicola infection in Octoploid strawberry( Fragaria × ananassa )
    Article Snippet: Briefly, total RNA was extracted from strawberry leaf samples, which included a mock inoculation and inoculation of the leaf samples at 24, 72, and 96 hpi using RNAiso Plus (TaKaRa, Otsu, Japan), followed by reverse transcription with an oligo(dT) primer using the Superscript III RT-PCR kit (Invitrogen, Carlsbad, CA).

    Article Title: Neopterin production in relation to COVID-19 in the Haut-Ogooué Province, Gabon.
    Article Snippet: The real-time RT-PCR assay was performed using a Superscript III RT-PCR kit Invitrogen in simplex, with primers and probes targeting the envelope coding for the E gene and the RdRP gene coding for RNA-dependent RNA polymerase (Tib-Molbiol kit).

    Article Title: Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations
    Article Snippet: RNA (1 μg) was reverse transcribed into cDNA libraries using Superscript III RT-PCR kit (Invitrogen; 18080-093), and qPCR was performed with QuantStudio3 (Applied Biosystems) using qPCR SYBR Master Mix (Promega; A6001) to detect SYBR Green 1.

    Article Title: Nanoparticle targeting of neutrophil glycolysis prevents lung ischemia-reperfusion injury.
    Article Snippet: Neutrophils exacerbate pulmonary ischemia-reperfusion injury (IRI) resulting in poor short and long-term outcomes for lung transplant recipients.. Glycolysis powers neutrophil activation, but it remains unclear if neutrophil-specific targeting of this pathway will inhibit IRI.. Lipid nanoparticles containing the glycolysis flux inhibitor 2-deoxyglucose (2-DG) were conjugated to neutrophil-specific Ly6G antibodies (NP-Ly6G[2-DG]).

    Article Title: Epidemiology of first cases of SARS-CoV-2 infection, from March to April 2020, in Gabon
    Article Snippet: RT-PCR was performed using Superscript III RT-PCR kit Invitrogen in simplex, primers and probes targeted the E gene coding envelope and the RdRP gene coding RNA-dependent RNA polymerase (kit Tib-Molbiol), in a 7500 Real Time PCR System (Applied Biosystems).

    Article Title: Arginase 1 is a marker of protection against illness in contacts of leprosy patients
    Article Snippet: Standard denaturing agarose gel electrophoresis was performed to determine RNA integrity visualized on a UV transilluminator. cDNA synthesis was carried out using the Superscript III RT-PCR Kit (Applied Biosystems, Branchburg, NJ, USA).

    Reverse Transcription:

    Article Title: Mesenchymal Stem Cell Exosomes as Immunomodulatory Therapy for Corneal Scarring
    Article Snippet: Reverse transcription of total RNA (1 μg) was performed with a Superscript III RT-PCR kit (Thermo Fisher).

    Article Title: Establishment of transgene-free induced pluripotent stem cells reprogrammed from human stem cells of apical papilla for neural differentiation
    Article Snippet: Total RNA was isolated using RNeasy Mini Kit (Qiagen, Vaenicia, CA, USA) from TF-SCAP iPSCs grown on Matrigel with TESR2 medium and neural-like cells after neurogenesis. cDNA was synthesized with a SuperScript III RT-PCR kit (Invitrogen) followed by PCR using Taq DNA Polymerase (Invitrogen) to detect the human neural gene expression listed in Table .

    Article Title: Genome-wide analysis of LysM gene family members and their expression in response to Colletotrichum fructicola infection in Octoploid strawberry( Fragaria × ananassa )
    Article Snippet: Briefly, total RNA was extracted from strawberry leaf samples, which included a mock inoculation and inoculation of the leaf samples at 24, 72, and 96 hpi using RNAiso Plus (TaKaRa, Otsu, Japan), followed by reverse transcription with an oligo(dT) primer using the Superscript III RT-PCR kit (Invitrogen, Carlsbad, CA).

    Article Title: Neopterin production in relation to COVID-19 in the Haut-Ogooué Province, Gabon.
    Article Snippet: The real-time RT-PCR assay was performed using a Superscript III RT-PCR kit Invitrogen in simplex, with primers and probes targeting the envelope coding for the E gene and the RdRP gene coding for RNA-dependent RNA polymerase (Tib-Molbiol kit).

    Article Title: Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations
    Article Snippet: RNA (1 μg) was reverse transcribed into cDNA libraries using Superscript III RT-PCR kit (Invitrogen; 18080-093), and qPCR was performed with QuantStudio3 (Applied Biosystems) using qPCR SYBR Master Mix (Promega; A6001) to detect SYBR Green 1.

    Article Title: Nanoparticle targeting of neutrophil glycolysis prevents lung ischemia-reperfusion injury.
    Article Snippet: Neutrophils exacerbate pulmonary ischemia-reperfusion injury (IRI) resulting in poor short and long-term outcomes for lung transplant recipients.. Glycolysis powers neutrophil activation, but it remains unclear if neutrophil-specific targeting of this pathway will inhibit IRI.. Lipid nanoparticles containing the glycolysis flux inhibitor 2-deoxyglucose (2-DG) were conjugated to neutrophil-specific Ly6G antibodies (NP-Ly6G[2-DG]).

    Article Title: Epidemiology of first cases of SARS-CoV-2 infection, from March to April 2020, in Gabon
    Article Snippet: RT-PCR was performed using Superscript III RT-PCR kit Invitrogen in simplex, primers and probes targeted the E gene coding envelope and the RdRP gene coding RNA-dependent RNA polymerase (kit Tib-Molbiol), in a 7500 Real Time PCR System (Applied Biosystems).

    Article Title: Arginase 1 is a marker of protection against illness in contacts of leprosy patients
    Article Snippet: Standard denaturing agarose gel electrophoresis was performed to determine RNA integrity visualized on a UV transilluminator. cDNA synthesis was carried out using the Superscript III RT-PCR Kit (Applied Biosystems, Branchburg, NJ, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Mesenchymal Stem Cell Exosomes as Immunomodulatory Therapy for Corneal Scarring
    Article Snippet: Reverse transcription of total RNA (1 μg) was performed with a Superscript III RT-PCR kit (Thermo Fisher).

    Article Title: Establishment of transgene-free induced pluripotent stem cells reprogrammed from human stem cells of apical papilla for neural differentiation
    Article Snippet: Total RNA was isolated using RNeasy Mini Kit (Qiagen, Vaenicia, CA, USA) from TF-SCAP iPSCs grown on Matrigel with TESR2 medium and neural-like cells after neurogenesis. cDNA was synthesized with a SuperScript III RT-PCR kit (Invitrogen) followed by PCR using Taq DNA Polymerase (Invitrogen) to detect the human neural gene expression listed in Table .

    Article Title: Genome-wide analysis of LysM gene family members and their expression in response to Colletotrichum fructicola infection in Octoploid strawberry( Fragaria × ananassa )
    Article Snippet: Briefly, total RNA was extracted from strawberry leaf samples, which included a mock inoculation and inoculation of the leaf samples at 24, 72, and 96 hpi using RNAiso Plus (TaKaRa, Otsu, Japan), followed by reverse transcription with an oligo(dT) primer using the Superscript III RT-PCR kit (Invitrogen, Carlsbad, CA).

    Article Title: Neopterin production in relation to COVID-19 in the Haut-Ogooué Province, Gabon.
    Article Snippet: The real-time RT-PCR assay was performed using a Superscript III RT-PCR kit Invitrogen in simplex, with primers and probes targeting the envelope coding for the E gene and the RdRP gene coding for RNA-dependent RNA polymerase (Tib-Molbiol kit).

    Article Title: Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations
    Article Snippet: RNA (1 μg) was reverse transcribed into cDNA libraries using Superscript III RT-PCR kit (Invitrogen; 18080-093), and qPCR was performed with QuantStudio3 (Applied Biosystems) using qPCR SYBR Master Mix (Promega; A6001) to detect SYBR Green 1.

    Article Title: Nanoparticle targeting of neutrophil glycolysis prevents lung ischemia-reperfusion injury.
    Article Snippet: Neutrophils exacerbate pulmonary ischemia-reperfusion injury (IRI) resulting in poor short and long-term outcomes for lung transplant recipients.. Glycolysis powers neutrophil activation, but it remains unclear if neutrophil-specific targeting of this pathway will inhibit IRI.. Lipid nanoparticles containing the glycolysis flux inhibitor 2-deoxyglucose (2-DG) were conjugated to neutrophil-specific Ly6G antibodies (NP-Ly6G[2-DG]).

    Article Title: Epidemiology of first cases of SARS-CoV-2 infection, from March to April 2020, in Gabon
    Article Snippet: RT-PCR was performed using Superscript III RT-PCR kit Invitrogen in simplex, primers and probes targeted the E gene coding envelope and the RdRP gene coding RNA-dependent RNA polymerase (kit Tib-Molbiol), in a 7500 Real Time PCR System (Applied Biosystems).

    Article Title: Arginase 1 is a marker of protection against illness in contacts of leprosy patients
    Article Snippet: Standard denaturing agarose gel electrophoresis was performed to determine RNA integrity visualized on a UV transilluminator. cDNA synthesis was carried out using the Superscript III RT-PCR Kit (Applied Biosystems, Branchburg, NJ, USA).

    SYBR Green Assay:

    Article Title: Mesenchymal Stem Cell Exosomes as Immunomodulatory Therapy for Corneal Scarring
    Article Snippet: Reverse transcription of total RNA (1 μg) was performed with a Superscript III RT-PCR kit (Thermo Fisher).

    Article Title: Establishment of transgene-free induced pluripotent stem cells reprogrammed from human stem cells of apical papilla for neural differentiation
    Article Snippet: Total RNA was isolated using RNeasy Mini Kit (Qiagen, Vaenicia, CA, USA) from TF-SCAP iPSCs grown on Matrigel with TESR2 medium and neural-like cells after neurogenesis. cDNA was synthesized with a SuperScript III RT-PCR kit (Invitrogen) followed by PCR using Taq DNA Polymerase (Invitrogen) to detect the human neural gene expression listed in Table .

    Article Title: Genome-wide analysis of LysM gene family members and their expression in response to Colletotrichum fructicola infection in Octoploid strawberry( Fragaria × ananassa )
    Article Snippet: Briefly, total RNA was extracted from strawberry leaf samples, which included a mock inoculation and inoculation of the leaf samples at 24, 72, and 96 hpi using RNAiso Plus (TaKaRa, Otsu, Japan), followed by reverse transcription with an oligo(dT) primer using the Superscript III RT-PCR kit (Invitrogen, Carlsbad, CA).

    Article Title: Neopterin production in relation to COVID-19 in the Haut-Ogooué Province, Gabon.
    Article Snippet: The real-time RT-PCR assay was performed using a Superscript III RT-PCR kit Invitrogen in simplex, with primers and probes targeting the envelope coding for the E gene and the RdRP gene coding for RNA-dependent RNA polymerase (Tib-Molbiol kit).

    Article Title: Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations
    Article Snippet: RNA (1 μg) was reverse transcribed into cDNA libraries using Superscript III RT-PCR kit (Invitrogen; 18080-093), and qPCR was performed with QuantStudio3 (Applied Biosystems) using qPCR SYBR Master Mix (Promega; A6001) to detect SYBR Green 1.

    Article Title: Nanoparticle targeting of neutrophil glycolysis prevents lung ischemia-reperfusion injury.
    Article Snippet: Neutrophils exacerbate pulmonary ischemia-reperfusion injury (IRI) resulting in poor short and long-term outcomes for lung transplant recipients.. Glycolysis powers neutrophil activation, but it remains unclear if neutrophil-specific targeting of this pathway will inhibit IRI.. Lipid nanoparticles containing the glycolysis flux inhibitor 2-deoxyglucose (2-DG) were conjugated to neutrophil-specific Ly6G antibodies (NP-Ly6G[2-DG]).

    Article Title: Epidemiology of first cases of SARS-CoV-2 infection, from March to April 2020, in Gabon
    Article Snippet: RT-PCR was performed using Superscript III RT-PCR kit Invitrogen in simplex, primers and probes targeted the E gene coding envelope and the RdRP gene coding RNA-dependent RNA polymerase (kit Tib-Molbiol), in a 7500 Real Time PCR System (Applied Biosystems).

    Article Title: Arginase 1 is a marker of protection against illness in contacts of leprosy patients
    Article Snippet: Standard denaturing agarose gel electrophoresis was performed to determine RNA integrity visualized on a UV transilluminator. cDNA synthesis was carried out using the Superscript III RT-PCR Kit (Applied Biosystems, Branchburg, NJ, USA).

    Isolation:

    Article Title: Mesenchymal Stem Cell Exosomes as Immunomodulatory Therapy for Corneal Scarring
    Article Snippet: Reverse transcription of total RNA (1 μg) was performed with a Superscript III RT-PCR kit (Thermo Fisher).

    Article Title: Establishment of transgene-free induced pluripotent stem cells reprogrammed from human stem cells of apical papilla for neural differentiation
    Article Snippet: Total RNA was isolated using RNeasy Mini Kit (Qiagen, Vaenicia, CA, USA) from TF-SCAP iPSCs grown on Matrigel with TESR2 medium and neural-like cells after neurogenesis. cDNA was synthesized with a SuperScript III RT-PCR kit (Invitrogen) followed by PCR using Taq DNA Polymerase (Invitrogen) to detect the human neural gene expression listed in Table .

    Article Title: Genome-wide analysis of LysM gene family members and their expression in response to Colletotrichum fructicola infection in Octoploid strawberry( Fragaria × ananassa )
    Article Snippet: Briefly, total RNA was extracted from strawberry leaf samples, which included a mock inoculation and inoculation of the leaf samples at 24, 72, and 96 hpi using RNAiso Plus (TaKaRa, Otsu, Japan), followed by reverse transcription with an oligo(dT) primer using the Superscript III RT-PCR kit (Invitrogen, Carlsbad, CA).

    Article Title: Neopterin production in relation to COVID-19 in the Haut-Ogooué Province, Gabon.
    Article Snippet: The real-time RT-PCR assay was performed using a Superscript III RT-PCR kit Invitrogen in simplex, with primers and probes targeting the envelope coding for the E gene and the RdRP gene coding for RNA-dependent RNA polymerase (Tib-Molbiol kit).

    Article Title: Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations
    Article Snippet: RNA (1 μg) was reverse transcribed into cDNA libraries using Superscript III RT-PCR kit (Invitrogen; 18080-093), and qPCR was performed with QuantStudio3 (Applied Biosystems) using qPCR SYBR Master Mix (Promega; A6001) to detect SYBR Green 1.

    Article Title: Nanoparticle targeting of neutrophil glycolysis prevents lung ischemia-reperfusion injury.
    Article Snippet: Neutrophils exacerbate pulmonary ischemia-reperfusion injury (IRI) resulting in poor short and long-term outcomes for lung transplant recipients.. Glycolysis powers neutrophil activation, but it remains unclear if neutrophil-specific targeting of this pathway will inhibit IRI.. Lipid nanoparticles containing the glycolysis flux inhibitor 2-deoxyglucose (2-DG) were conjugated to neutrophil-specific Ly6G antibodies (NP-Ly6G[2-DG]).

    Article Title: Epidemiology of first cases of SARS-CoV-2 infection, from March to April 2020, in Gabon
    Article Snippet: RT-PCR was performed using Superscript III RT-PCR kit Invitrogen in simplex, primers and probes targeted the E gene coding envelope and the RdRP gene coding RNA-dependent RNA polymerase (kit Tib-Molbiol), in a 7500 Real Time PCR System (Applied Biosystems).

    Article Title: Arginase 1 is a marker of protection against illness in contacts of leprosy patients
    Article Snippet: Standard denaturing agarose gel electrophoresis was performed to determine RNA integrity visualized on a UV transilluminator. cDNA synthesis was carried out using the Superscript III RT-PCR Kit (Applied Biosystems, Branchburg, NJ, USA).

    Synthesized:

    Article Title: Mesenchymal Stem Cell Exosomes as Immunomodulatory Therapy for Corneal Scarring
    Article Snippet: Reverse transcription of total RNA (1 μg) was performed with a Superscript III RT-PCR kit (Thermo Fisher).

    Article Title: Establishment of transgene-free induced pluripotent stem cells reprogrammed from human stem cells of apical papilla for neural differentiation
    Article Snippet: Total RNA was isolated using RNeasy Mini Kit (Qiagen, Vaenicia, CA, USA) from TF-SCAP iPSCs grown on Matrigel with TESR2 medium and neural-like cells after neurogenesis. cDNA was synthesized with a SuperScript III RT-PCR kit (Invitrogen) followed by PCR using Taq DNA Polymerase (Invitrogen) to detect the human neural gene expression listed in Table .

    Article Title: Genome-wide analysis of LysM gene family members and their expression in response to Colletotrichum fructicola infection in Octoploid strawberry( Fragaria × ananassa )
    Article Snippet: Briefly, total RNA was extracted from strawberry leaf samples, which included a mock inoculation and inoculation of the leaf samples at 24, 72, and 96 hpi using RNAiso Plus (TaKaRa, Otsu, Japan), followed by reverse transcription with an oligo(dT) primer using the Superscript III RT-PCR kit (Invitrogen, Carlsbad, CA).

    Article Title: Neopterin production in relation to COVID-19 in the Haut-Ogooué Province, Gabon.
    Article Snippet: The real-time RT-PCR assay was performed using a Superscript III RT-PCR kit Invitrogen in simplex, with primers and probes targeting the envelope coding for the E gene and the RdRP gene coding for RNA-dependent RNA polymerase (Tib-Molbiol kit).

    Article Title: Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations
    Article Snippet: RNA (1 μg) was reverse transcribed into cDNA libraries using Superscript III RT-PCR kit (Invitrogen; 18080-093), and qPCR was performed with QuantStudio3 (Applied Biosystems) using qPCR SYBR Master Mix (Promega; A6001) to detect SYBR Green 1.

    Article Title: Nanoparticle targeting of neutrophil glycolysis prevents lung ischemia-reperfusion injury.
    Article Snippet: Neutrophils exacerbate pulmonary ischemia-reperfusion injury (IRI) resulting in poor short and long-term outcomes for lung transplant recipients.. Glycolysis powers neutrophil activation, but it remains unclear if neutrophil-specific targeting of this pathway will inhibit IRI.. Lipid nanoparticles containing the glycolysis flux inhibitor 2-deoxyglucose (2-DG) were conjugated to neutrophil-specific Ly6G antibodies (NP-Ly6G[2-DG]).

    Article Title: Epidemiology of first cases of SARS-CoV-2 infection, from March to April 2020, in Gabon
    Article Snippet: RT-PCR was performed using Superscript III RT-PCR kit Invitrogen in simplex, primers and probes targeted the E gene coding envelope and the RdRP gene coding RNA-dependent RNA polymerase (kit Tib-Molbiol), in a 7500 Real Time PCR System (Applied Biosystems).

    Article Title: Arginase 1 is a marker of protection against illness in contacts of leprosy patients
    Article Snippet: Standard denaturing agarose gel electrophoresis was performed to determine RNA integrity visualized on a UV transilluminator. cDNA synthesis was carried out using the Superscript III RT-PCR Kit (Applied Biosystems, Branchburg, NJ, USA).

    Polymerase Chain Reaction:

    Article Title: Mesenchymal Stem Cell Exosomes as Immunomodulatory Therapy for Corneal Scarring
    Article Snippet: Reverse transcription of total RNA (1 μg) was performed with a Superscript III RT-PCR kit (Thermo Fisher).

    Article Title: Establishment of transgene-free induced pluripotent stem cells reprogrammed from human stem cells of apical papilla for neural differentiation
    Article Snippet: Total RNA was isolated using RNeasy Mini Kit (Qiagen, Vaenicia, CA, USA) from TF-SCAP iPSCs grown on Matrigel with TESR2 medium and neural-like cells after neurogenesis. cDNA was synthesized with a SuperScript III RT-PCR kit (Invitrogen) followed by PCR using Taq DNA Polymerase (Invitrogen) to detect the human neural gene expression listed in Table .

    Article Title: Genome-wide analysis of LysM gene family members and their expression in response to Colletotrichum fructicola infection in Octoploid strawberry( Fragaria × ananassa )
    Article Snippet: Briefly, total RNA was extracted from strawberry leaf samples, which included a mock inoculation and inoculation of the leaf samples at 24, 72, and 96 hpi using RNAiso Plus (TaKaRa, Otsu, Japan), followed by reverse transcription with an oligo(dT) primer using the Superscript III RT-PCR kit (Invitrogen, Carlsbad, CA).

    Article Title: Neopterin production in relation to COVID-19 in the Haut-Ogooué Province, Gabon.
    Article Snippet: The real-time RT-PCR assay was performed using a Superscript III RT-PCR kit Invitrogen in simplex, with primers and probes targeting the envelope coding for the E gene and the RdRP gene coding for RNA-dependent RNA polymerase (Tib-Molbiol kit).

    Article Title: Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations
    Article Snippet: RNA (1 μg) was reverse transcribed into cDNA libraries using Superscript III RT-PCR kit (Invitrogen; 18080-093), and qPCR was performed with QuantStudio3 (Applied Biosystems) using qPCR SYBR Master Mix (Promega; A6001) to detect SYBR Green 1.

    Article Title: Nanoparticle targeting of neutrophil glycolysis prevents lung ischemia-reperfusion injury.
    Article Snippet: Neutrophils exacerbate pulmonary ischemia-reperfusion injury (IRI) resulting in poor short and long-term outcomes for lung transplant recipients.. Glycolysis powers neutrophil activation, but it remains unclear if neutrophil-specific targeting of this pathway will inhibit IRI.. Lipid nanoparticles containing the glycolysis flux inhibitor 2-deoxyglucose (2-DG) were conjugated to neutrophil-specific Ly6G antibodies (NP-Ly6G[2-DG]).

    Article Title: Epidemiology of first cases of SARS-CoV-2 infection, from March to April 2020, in Gabon
    Article Snippet: RT-PCR was performed using Superscript III RT-PCR kit Invitrogen in simplex, primers and probes targeted the E gene coding envelope and the RdRP gene coding RNA-dependent RNA polymerase (kit Tib-Molbiol), in a 7500 Real Time PCR System (Applied Biosystems).

    Article Title: Arginase 1 is a marker of protection against illness in contacts of leprosy patients
    Article Snippet: Standard denaturing agarose gel electrophoresis was performed to determine RNA integrity visualized on a UV transilluminator. cDNA synthesis was carried out using the Superscript III RT-PCR Kit (Applied Biosystems, Branchburg, NJ, USA).

    Plasmid Preparation:

    Article Title: Mesenchymal Stem Cell Exosomes as Immunomodulatory Therapy for Corneal Scarring
    Article Snippet: Reverse transcription of total RNA (1 μg) was performed with a Superscript III RT-PCR kit (Thermo Fisher).

    Article Title: Establishment of transgene-free induced pluripotent stem cells reprogrammed from human stem cells of apical papilla for neural differentiation
    Article Snippet: Total RNA was isolated using RNeasy Mini Kit (Qiagen, Vaenicia, CA, USA) from TF-SCAP iPSCs grown on Matrigel with TESR2 medium and neural-like cells after neurogenesis. cDNA was synthesized with a SuperScript III RT-PCR kit (Invitrogen) followed by PCR using Taq DNA Polymerase (Invitrogen) to detect the human neural gene expression listed in Table .

    Article Title: Genome-wide analysis of LysM gene family members and their expression in response to Colletotrichum fructicola infection in Octoploid strawberry( Fragaria × ananassa )
    Article Snippet: Briefly, total RNA was extracted from strawberry leaf samples, which included a mock inoculation and inoculation of the leaf samples at 24, 72, and 96 hpi using RNAiso Plus (TaKaRa, Otsu, Japan), followed by reverse transcription with an oligo(dT) primer using the Superscript III RT-PCR kit (Invitrogen, Carlsbad, CA).

    Article Title: Neopterin production in relation to COVID-19 in the Haut-Ogooué Province, Gabon.
    Article Snippet: The real-time RT-PCR assay was performed using a Superscript III RT-PCR kit Invitrogen in simplex, with primers and probes targeting the envelope coding for the E gene and the RdRP gene coding for RNA-dependent RNA polymerase (Tib-Molbiol kit).

    Article Title: Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations
    Article Snippet: RNA (1 μg) was reverse transcribed into cDNA libraries using Superscript III RT-PCR kit (Invitrogen; 18080-093), and qPCR was performed with QuantStudio3 (Applied Biosystems) using qPCR SYBR Master Mix (Promega; A6001) to detect SYBR Green 1.

    Article Title: Nanoparticle targeting of neutrophil glycolysis prevents lung ischemia-reperfusion injury.
    Article Snippet: Neutrophils exacerbate pulmonary ischemia-reperfusion injury (IRI) resulting in poor short and long-term outcomes for lung transplant recipients.. Glycolysis powers neutrophil activation, but it remains unclear if neutrophil-specific targeting of this pathway will inhibit IRI.. Lipid nanoparticles containing the glycolysis flux inhibitor 2-deoxyglucose (2-DG) were conjugated to neutrophil-specific Ly6G antibodies (NP-Ly6G[2-DG]).

    Article Title: Epidemiology of first cases of SARS-CoV-2 infection, from March to April 2020, in Gabon
    Article Snippet: RT-PCR was performed using Superscript III RT-PCR kit Invitrogen in simplex, primers and probes targeted the E gene coding envelope and the RdRP gene coding RNA-dependent RNA polymerase (kit Tib-Molbiol), in a 7500 Real Time PCR System (Applied Biosystems).

    Article Title: Arginase 1 is a marker of protection against illness in contacts of leprosy patients
    Article Snippet: Standard denaturing agarose gel electrophoresis was performed to determine RNA integrity visualized on a UV transilluminator. cDNA synthesis was carried out using the Superscript III RT-PCR Kit (Applied Biosystems, Branchburg, NJ, USA).

    Transduction:

    Article Title: Mesenchymal Stem Cell Exosomes as Immunomodulatory Therapy for Corneal Scarring
    Article Snippet: Reverse transcription of total RNA (1 μg) was performed with a Superscript III RT-PCR kit (Thermo Fisher).

    Article Title: Establishment of transgene-free induced pluripotent stem cells reprogrammed from human stem cells of apical papilla for neural differentiation
    Article Snippet: Total RNA was isolated using RNeasy Mini Kit (Qiagen, Vaenicia, CA, USA) from TF-SCAP iPSCs grown on Matrigel with TESR2 medium and neural-like cells after neurogenesis. cDNA was synthesized with a SuperScript III RT-PCR kit (Invitrogen) followed by PCR using Taq DNA Polymerase (Invitrogen) to detect the human neural gene expression listed in Table .

    Article Title: Genome-wide analysis of LysM gene family members and their expression in response to Colletotrichum fructicola infection in Octoploid strawberry( Fragaria × ananassa )
    Article Snippet: Briefly, total RNA was extracted from strawberry leaf samples, which included a mock inoculation and inoculation of the leaf samples at 24, 72, and 96 hpi using RNAiso Plus (TaKaRa, Otsu, Japan), followed by reverse transcription with an oligo(dT) primer using the Superscript III RT-PCR kit (Invitrogen, Carlsbad, CA).

    Article Title: Neopterin production in relation to COVID-19 in the Haut-Ogooué Province, Gabon.
    Article Snippet: The real-time RT-PCR assay was performed using a Superscript III RT-PCR kit Invitrogen in simplex, with primers and probes targeting the envelope coding for the E gene and the RdRP gene coding for RNA-dependent RNA polymerase (Tib-Molbiol kit).

    Article Title: Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations
    Article Snippet: RNA (1 μg) was reverse transcribed into cDNA libraries using Superscript III RT-PCR kit (Invitrogen; 18080-093), and qPCR was performed with QuantStudio3 (Applied Biosystems) using qPCR SYBR Master Mix (Promega; A6001) to detect SYBR Green 1.

    Article Title: Nanoparticle targeting of neutrophil glycolysis prevents lung ischemia-reperfusion injury.
    Article Snippet: Neutrophils exacerbate pulmonary ischemia-reperfusion injury (IRI) resulting in poor short and long-term outcomes for lung transplant recipients.. Glycolysis powers neutrophil activation, but it remains unclear if neutrophil-specific targeting of this pathway will inhibit IRI.. Lipid nanoparticles containing the glycolysis flux inhibitor 2-deoxyglucose (2-DG) were conjugated to neutrophil-specific Ly6G antibodies (NP-Ly6G[2-DG]).

    Article Title: Epidemiology of first cases of SARS-CoV-2 infection, from March to April 2020, in Gabon
    Article Snippet: RT-PCR was performed using Superscript III RT-PCR kit Invitrogen in simplex, primers and probes targeted the E gene coding envelope and the RdRP gene coding RNA-dependent RNA polymerase (kit Tib-Molbiol), in a 7500 Real Time PCR System (Applied Biosystems).

    Article Title: Arginase 1 is a marker of protection against illness in contacts of leprosy patients
    Article Snippet: Standard denaturing agarose gel electrophoresis was performed to determine RNA integrity visualized on a UV transilluminator. cDNA synthesis was carried out using the Superscript III RT-PCR Kit (Applied Biosystems, Branchburg, NJ, USA).

    Transfection:

    Article Title: Mesenchymal Stem Cell Exosomes as Immunomodulatory Therapy for Corneal Scarring
    Article Snippet: Reverse transcription of total RNA (1 μg) was performed with a Superscript III RT-PCR kit (Thermo Fisher).

    Article Title: Establishment of transgene-free induced pluripotent stem cells reprogrammed from human stem cells of apical papilla for neural differentiation
    Article Snippet: Total RNA was isolated using RNeasy Mini Kit (Qiagen, Vaenicia, CA, USA) from TF-SCAP iPSCs grown on Matrigel with TESR2 medium and neural-like cells after neurogenesis. cDNA was synthesized with a SuperScript III RT-PCR kit (Invitrogen) followed by PCR using Taq DNA Polymerase (Invitrogen) to detect the human neural gene expression listed in Table .

    Article Title: Genome-wide analysis of LysM gene family members and their expression in response to Colletotrichum fructicola infection in Octoploid strawberry( Fragaria × ananassa )
    Article Snippet: Briefly, total RNA was extracted from strawberry leaf samples, which included a mock inoculation and inoculation of the leaf samples at 24, 72, and 96 hpi using RNAiso Plus (TaKaRa, Otsu, Japan), followed by reverse transcription with an oligo(dT) primer using the Superscript III RT-PCR kit (Invitrogen, Carlsbad, CA).

    Article Title: Neopterin production in relation to COVID-19 in the Haut-Ogooué Province, Gabon.
    Article Snippet: The real-time RT-PCR assay was performed using a Superscript III RT-PCR kit Invitrogen in simplex, with primers and probes targeting the envelope coding for the E gene and the RdRP gene coding for RNA-dependent RNA polymerase (Tib-Molbiol kit).

    Article Title: Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations
    Article Snippet: RNA (1 μg) was reverse transcribed into cDNA libraries using Superscript III RT-PCR kit (Invitrogen; 18080-093), and qPCR was performed with QuantStudio3 (Applied Biosystems) using qPCR SYBR Master Mix (Promega; A6001) to detect SYBR Green 1.

    Article Title: Nanoparticle targeting of neutrophil glycolysis prevents lung ischemia-reperfusion injury.
    Article Snippet: Neutrophils exacerbate pulmonary ischemia-reperfusion injury (IRI) resulting in poor short and long-term outcomes for lung transplant recipients.. Glycolysis powers neutrophil activation, but it remains unclear if neutrophil-specific targeting of this pathway will inhibit IRI.. Lipid nanoparticles containing the glycolysis flux inhibitor 2-deoxyglucose (2-DG) were conjugated to neutrophil-specific Ly6G antibodies (NP-Ly6G[2-DG]).

    Article Title: Epidemiology of first cases of SARS-CoV-2 infection, from March to April 2020, in Gabon
    Article Snippet: RT-PCR was performed using Superscript III RT-PCR kit Invitrogen in simplex, primers and probes targeted the E gene coding envelope and the RdRP gene coding RNA-dependent RNA polymerase (kit Tib-Molbiol), in a 7500 Real Time PCR System (Applied Biosystems).

    Article Title: Arginase 1 is a marker of protection against illness in contacts of leprosy patients
    Article Snippet: Standard denaturing agarose gel electrophoresis was performed to determine RNA integrity visualized on a UV transilluminator. cDNA synthesis was carried out using the Superscript III RT-PCR Kit (Applied Biosystems, Branchburg, NJ, USA).

    Selection:

    Article Title: Mesenchymal Stem Cell Exosomes as Immunomodulatory Therapy for Corneal Scarring
    Article Snippet: Reverse transcription of total RNA (1 μg) was performed with a Superscript III RT-PCR kit (Thermo Fisher).

    Article Title: Establishment of transgene-free induced pluripotent stem cells reprogrammed from human stem cells of apical papilla for neural differentiation
    Article Snippet: Total RNA was isolated using RNeasy Mini Kit (Qiagen, Vaenicia, CA, USA) from TF-SCAP iPSCs grown on Matrigel with TESR2 medium and neural-like cells after neurogenesis. cDNA was synthesized with a SuperScript III RT-PCR kit (Invitrogen) followed by PCR using Taq DNA Polymerase (Invitrogen) to detect the human neural gene expression listed in Table .

    Article Title: Genome-wide analysis of LysM gene family members and their expression in response to Colletotrichum fructicola infection in Octoploid strawberry( Fragaria × ananassa )
    Article Snippet: Briefly, total RNA was extracted from strawberry leaf samples, which included a mock inoculation and inoculation of the leaf samples at 24, 72, and 96 hpi using RNAiso Plus (TaKaRa, Otsu, Japan), followed by reverse transcription with an oligo(dT) primer using the Superscript III RT-PCR kit (Invitrogen, Carlsbad, CA).

    Article Title: Neopterin production in relation to COVID-19 in the Haut-Ogooué Province, Gabon.
    Article Snippet: The real-time RT-PCR assay was performed using a Superscript III RT-PCR kit Invitrogen in simplex, with primers and probes targeting the envelope coding for the E gene and the RdRP gene coding for RNA-dependent RNA polymerase (Tib-Molbiol kit).

    Article Title: Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations
    Article Snippet: RNA (1 μg) was reverse transcribed into cDNA libraries using Superscript III RT-PCR kit (Invitrogen; 18080-093), and qPCR was performed with QuantStudio3 (Applied Biosystems) using qPCR SYBR Master Mix (Promega; A6001) to detect SYBR Green 1.

    Article Title: Nanoparticle targeting of neutrophil glycolysis prevents lung ischemia-reperfusion injury.
    Article Snippet: Neutrophils exacerbate pulmonary ischemia-reperfusion injury (IRI) resulting in poor short and long-term outcomes for lung transplant recipients.. Glycolysis powers neutrophil activation, but it remains unclear if neutrophil-specific targeting of this pathway will inhibit IRI.. Lipid nanoparticles containing the glycolysis flux inhibitor 2-deoxyglucose (2-DG) were conjugated to neutrophil-specific Ly6G antibodies (NP-Ly6G[2-DG]).

    Article Title: Epidemiology of first cases of SARS-CoV-2 infection, from March to April 2020, in Gabon
    Article Snippet: RT-PCR was performed using Superscript III RT-PCR kit Invitrogen in simplex, primers and probes targeted the E gene coding envelope and the RdRP gene coding RNA-dependent RNA polymerase (kit Tib-Molbiol), in a 7500 Real Time PCR System (Applied Biosystems).

    Article Title: Arginase 1 is a marker of protection against illness in contacts of leprosy patients
    Article Snippet: Standard denaturing agarose gel electrophoresis was performed to determine RNA integrity visualized on a UV transilluminator. cDNA synthesis was carried out using the Superscript III RT-PCR Kit (Applied Biosystems, Branchburg, NJ, USA).

    Clone Assay:

    Article Title: Mesenchymal Stem Cell Exosomes as Immunomodulatory Therapy for Corneal Scarring
    Article Snippet: Reverse transcription of total RNA (1 μg) was performed with a Superscript III RT-PCR kit (Thermo Fisher).

    Article Title: Establishment of transgene-free induced pluripotent stem cells reprogrammed from human stem cells of apical papilla for neural differentiation
    Article Snippet: Total RNA was isolated using RNeasy Mini Kit (Qiagen, Vaenicia, CA, USA) from TF-SCAP iPSCs grown on Matrigel with TESR2 medium and neural-like cells after neurogenesis. cDNA was synthesized with a SuperScript III RT-PCR kit (Invitrogen) followed by PCR using Taq DNA Polymerase (Invitrogen) to detect the human neural gene expression listed in Table .

    Article Title: Genome-wide analysis of LysM gene family members and their expression in response to Colletotrichum fructicola infection in Octoploid strawberry( Fragaria × ananassa )
    Article Snippet: Briefly, total RNA was extracted from strawberry leaf samples, which included a mock inoculation and inoculation of the leaf samples at 24, 72, and 96 hpi using RNAiso Plus (TaKaRa, Otsu, Japan), followed by reverse transcription with an oligo(dT) primer using the Superscript III RT-PCR kit (Invitrogen, Carlsbad, CA).

    Article Title: Neopterin production in relation to COVID-19 in the Haut-Ogooué Province, Gabon.
    Article Snippet: The real-time RT-PCR assay was performed using a Superscript III RT-PCR kit Invitrogen in simplex, with primers and probes targeting the envelope coding for the E gene and the RdRP gene coding for RNA-dependent RNA polymerase (Tib-Molbiol kit).

    Article Title: Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations
    Article Snippet: RNA (1 μg) was reverse transcribed into cDNA libraries using Superscript III RT-PCR kit (Invitrogen; 18080-093), and qPCR was performed with QuantStudio3 (Applied Biosystems) using qPCR SYBR Master Mix (Promega; A6001) to detect SYBR Green 1.

    Article Title: Nanoparticle targeting of neutrophil glycolysis prevents lung ischemia-reperfusion injury.
    Article Snippet: Neutrophils exacerbate pulmonary ischemia-reperfusion injury (IRI) resulting in poor short and long-term outcomes for lung transplant recipients.. Glycolysis powers neutrophil activation, but it remains unclear if neutrophil-specific targeting of this pathway will inhibit IRI.. Lipid nanoparticles containing the glycolysis flux inhibitor 2-deoxyglucose (2-DG) were conjugated to neutrophil-specific Ly6G antibodies (NP-Ly6G[2-DG]).

    Article Title: Epidemiology of first cases of SARS-CoV-2 infection, from March to April 2020, in Gabon
    Article Snippet: RT-PCR was performed using Superscript III RT-PCR kit Invitrogen in simplex, primers and probes targeted the E gene coding envelope and the RdRP gene coding RNA-dependent RNA polymerase (kit Tib-Molbiol), in a 7500 Real Time PCR System (Applied Biosystems).

    Article Title: Arginase 1 is a marker of protection against illness in contacts of leprosy patients
    Article Snippet: Standard denaturing agarose gel electrophoresis was performed to determine RNA integrity visualized on a UV transilluminator. cDNA synthesis was carried out using the Superscript III RT-PCR Kit (Applied Biosystems, Branchburg, NJ, USA).

    Derivative Assay:

    Article Title: Mesenchymal Stem Cell Exosomes as Immunomodulatory Therapy for Corneal Scarring
    Article Snippet: Reverse transcription of total RNA (1 μg) was performed with a Superscript III RT-PCR kit (Thermo Fisher).

    Article Title: Establishment of transgene-free induced pluripotent stem cells reprogrammed from human stem cells of apical papilla for neural differentiation
    Article Snippet: Total RNA was isolated using RNeasy Mini Kit (Qiagen, Vaenicia, CA, USA) from TF-SCAP iPSCs grown on Matrigel with TESR2 medium and neural-like cells after neurogenesis. cDNA was synthesized with a SuperScript III RT-PCR kit (Invitrogen) followed by PCR using Taq DNA Polymerase (Invitrogen) to detect the human neural gene expression listed in Table .

    Article Title: Genome-wide analysis of LysM gene family members and their expression in response to Colletotrichum fructicola infection in Octoploid strawberry( Fragaria × ananassa )
    Article Snippet: Briefly, total RNA was extracted from strawberry leaf samples, which included a mock inoculation and inoculation of the leaf samples at 24, 72, and 96 hpi using RNAiso Plus (TaKaRa, Otsu, Japan), followed by reverse transcription with an oligo(dT) primer using the Superscript III RT-PCR kit (Invitrogen, Carlsbad, CA).

    Article Title: Neopterin production in relation to COVID-19 in the Haut-Ogooué Province, Gabon.
    Article Snippet: The real-time RT-PCR assay was performed using a Superscript III RT-PCR kit Invitrogen in simplex, with primers and probes targeting the envelope coding for the E gene and the RdRP gene coding for RNA-dependent RNA polymerase (Tib-Molbiol kit).

    Article Title: Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations
    Article Snippet: RNA (1 μg) was reverse transcribed into cDNA libraries using Superscript III RT-PCR kit (Invitrogen; 18080-093), and qPCR was performed with QuantStudio3 (Applied Biosystems) using qPCR SYBR Master Mix (Promega; A6001) to detect SYBR Green 1.

    Article Title: Nanoparticle targeting of neutrophil glycolysis prevents lung ischemia-reperfusion injury.
    Article Snippet: Neutrophils exacerbate pulmonary ischemia-reperfusion injury (IRI) resulting in poor short and long-term outcomes for lung transplant recipients.. Glycolysis powers neutrophil activation, but it remains unclear if neutrophil-specific targeting of this pathway will inhibit IRI.. Lipid nanoparticles containing the glycolysis flux inhibitor 2-deoxyglucose (2-DG) were conjugated to neutrophil-specific Ly6G antibodies (NP-Ly6G[2-DG]).

    Article Title: Epidemiology of first cases of SARS-CoV-2 infection, from March to April 2020, in Gabon
    Article Snippet: RT-PCR was performed using Superscript III RT-PCR kit Invitrogen in simplex, primers and probes targeted the E gene coding envelope and the RdRP gene coding RNA-dependent RNA polymerase (kit Tib-Molbiol), in a 7500 Real Time PCR System (Applied Biosystems).

    Article Title: Arginase 1 is a marker of protection against illness in contacts of leprosy patients
    Article Snippet: Standard denaturing agarose gel electrophoresis was performed to determine RNA integrity visualized on a UV transilluminator. cDNA synthesis was carried out using the Superscript III RT-PCR Kit (Applied Biosystems, Branchburg, NJ, USA).

    Agarose Gel Electrophoresis:

    Article Title: Mesenchymal Stem Cell Exosomes as Immunomodulatory Therapy for Corneal Scarring
    Article Snippet: Reverse transcription of total RNA (1 μg) was performed with a Superscript III RT-PCR kit (Thermo Fisher).

    Article Title: Establishment of transgene-free induced pluripotent stem cells reprogrammed from human stem cells of apical papilla for neural differentiation
    Article Snippet: Total RNA was isolated using RNeasy Mini Kit (Qiagen, Vaenicia, CA, USA) from TF-SCAP iPSCs grown on Matrigel with TESR2 medium and neural-like cells after neurogenesis. cDNA was synthesized with a SuperScript III RT-PCR kit (Invitrogen) followed by PCR using Taq DNA Polymerase (Invitrogen) to detect the human neural gene expression listed in Table .

    Article Title: Genome-wide analysis of LysM gene family members and their expression in response to Colletotrichum fructicola infection in Octoploid strawberry( Fragaria × ananassa )
    Article Snippet: Briefly, total RNA was extracted from strawberry leaf samples, which included a mock inoculation and inoculation of the leaf samples at 24, 72, and 96 hpi using RNAiso Plus (TaKaRa, Otsu, Japan), followed by reverse transcription with an oligo(dT) primer using the Superscript III RT-PCR kit (Invitrogen, Carlsbad, CA).

    Article Title: Neopterin production in relation to COVID-19 in the Haut-Ogooué Province, Gabon.
    Article Snippet: The real-time RT-PCR assay was performed using a Superscript III RT-PCR kit Invitrogen in simplex, with primers and probes targeting the envelope coding for the E gene and the RdRP gene coding for RNA-dependent RNA polymerase (Tib-Molbiol kit).

    Article Title: Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations
    Article Snippet: RNA (1 μg) was reverse transcribed into cDNA libraries using Superscript III RT-PCR kit (Invitrogen; 18080-093), and qPCR was performed with QuantStudio3 (Applied Biosystems) using qPCR SYBR Master Mix (Promega; A6001) to detect SYBR Green 1.

    Article Title: Nanoparticle targeting of neutrophil glycolysis prevents lung ischemia-reperfusion injury.
    Article Snippet: Neutrophils exacerbate pulmonary ischemia-reperfusion injury (IRI) resulting in poor short and long-term outcomes for lung transplant recipients.. Glycolysis powers neutrophil activation, but it remains unclear if neutrophil-specific targeting of this pathway will inhibit IRI.. Lipid nanoparticles containing the glycolysis flux inhibitor 2-deoxyglucose (2-DG) were conjugated to neutrophil-specific Ly6G antibodies (NP-Ly6G[2-DG]).

    Article Title: Epidemiology of first cases of SARS-CoV-2 infection, from March to April 2020, in Gabon
    Article Snippet: RT-PCR was performed using Superscript III RT-PCR kit Invitrogen in simplex, primers and probes targeted the E gene coding envelope and the RdRP gene coding RNA-dependent RNA polymerase (kit Tib-Molbiol), in a 7500 Real Time PCR System (Applied Biosystems).

    Article Title: Arginase 1 is a marker of protection against illness in contacts of leprosy patients
    Article Snippet: Standard denaturing agarose gel electrophoresis was performed to determine RNA integrity visualized on a UV transilluminator. cDNA synthesis was carried out using the Superscript III RT-PCR Kit (Applied Biosystems, Branchburg, NJ, USA).



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